Plant Isoprenoids: Methods and Protocols

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Divided into four convenient sections to better cover strategic areas in plant isoprenoid research, topics include measurement of core enzyme activities involved in the production of isoprenoid precursors, targeted analysis of major groups of isoprenoid metabolites, isoprenoid profiling in specialized organs such as trichomes and oil glands, as well as genetic, pharmacological, and bioinformatic tools that are particularly useful for plant molecular biologists.


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Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Isoprenoids: Methods and Protocols will serve as an excellent reference material that can be adapted to develop customized methods for different needs making the world of plant isoprenoids more accessible for all researchers.

Divided into four convenient sections to better cover strategic areas in plant isoprenoid research, topics include measurement of core enzyme activities involved in the production of isoprenoid precursors, targeted analysis of major groups of isoprenoid metabolites, isoprenoid profiling in specialized organs such as trichomes and oil glands as well as genetic, pharmacological and bioinformatic tools that are particularly useful for plant molecular biologists.

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Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Read more Read less. Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase. Increased and altered fragrance of tobacco plants after metabolic engineering using three monoterpene synthases from lemon. Mahmoud, S.

Metabolic engineering of essential oil yield and composition in mint by altering expression of deoxyxylulose phosphate reductoisomerase and menthofuran synthase. Manavalan, L. RNAi-mediated disruption of squalene synthase improves drought tolerance and yield in rice. McGarvey, D. Terpenoid metabolism. Plant Cell 7, — Munoz-Bertomeu, J. Up-regulation of 1-deoxy-D-xylulosephosphate synthase enhances production of essential oils in transgenic spike lavender.

Nielsen, M. Microbial synthesis of the forskolin precursor manoyl oxide in enantiomerically pure form. Nieto, B. Arabidopsis 3-hydroxymethylglutaryl-CoA reductase is regulated at the post-translational level in response to alterations of the sphingolipid and the sterol biosynthetic pathways. Phytochemistry 70, 53— Ohara, K. Limonene production in tobacco with Perilla limonene synthase cDNA. Ohto, C. Okada, A. Elicitor induced activation of the methylerythritol phosphate pathway toward phytoalexins biosynthesis in rice. Okada, K. Genetic evidence for the role of isopentenyl diphosphate isomerases in the mevalonate pathway and plant development in Arabidopsis.

Plant Cell Physiol. Paddon, C. High-level semi-synthetic production of the potent antimalarial artemisinin. Nature , — Page, J. Functional analysis of the final steps of the 1-Deoxy-D-xylulose 5-phosphate DXP pathway to isoprenoids in plants using virus-induced gene silencing. Pan, X. PhD thesis, University of Copenhagen.

Pateraki, I. Manoyl oxide 13R , the biosynthetic precursor of forskolin, is synthesized in specialized root cork cells in Coleus forskohlii. Berlin; Heidelberg: Springer. Google Scholar. Pickel, B. Identification and characterization of a kunzeaol synthase from Thapsia garganica : implications for the biosynthesis of the pharmaceutical thapsigargin. Ro, D. Production of the antimalarial drug precursor artemisinic acid in engineered yeast.

Robert, C.

Genetically engineered maize plants reveal distinct costs and benefits of constitutive volatile emissions in the field. Rodriguez-Concepcion, M.

Plant Isoprenoids : Methods and Protocols - ophvewcahelthe.cf

Regulation of carotenoid biosynthesis in plants: evidence for a key role of hydroxymethylbutenyl diphosphate reductase in controlling the supply of plastidial isoprenoid precursors. Rohmer, M. Isoprenoid biosynthesis in bacteria: a novel pathway for the early steps leading to isopentenyl diphosphate. Sallaud, C. A novel pathway for sesquiterpene biosynthesis from Z,Z-farnesyl pyrophosphate in the wild tomato Solanum habrochaites.

Plant Cell 21, — Sanmiya, K. Localization of farnesyl diphosphate synthase in chloroplasts. Shuiqin, W. Redirection of cytosolic or plastidic isoprenoid precursors elevates terpene production in plants.

Plant Isoprenoids : Methods and Protocols

Simonsen, H. Perspectives on using Physcomitrella patens as an alternative production platform for thapsigargin and other terpenoid drug candidates. Sommer, S. Intracellular localization of geranylpyrophosphate synthase from cell cultures of Lithospermum erythrorhizon. Phytochemistry 38, — Song, A. Overexpressing 3-hydroxymethylglutaryl coenzyme A reductase HMGR in the lactococcal mevalonate pathway for heterologous plant sesquiterpene production. Stermer, B. Regulation of Hmg-Coa reductase-activity in plants.

Lipid Res. Takahashi, S. Metabolic engineering of sesquiterpene metabolism in yeast.

Plant Genomics: Methods and Protocols

Ting, H. The metabolite chemotype of Nicotiana benthamiana transiently expressing artemisinin biosynthetic pathway genes is a function of CYP71AV1 type and relative gene dosage. New Phytol. Tsuruta, H.

Stanford Libraries

High-level production of amorpha-4,diene, a precursor of the antimalarial agent artemisinin, in Escherichia coli. Vaccaro, M. Plant Cell Tissue Organ Cult. Van Herpen, T. Nicotiana benthamiana as a production platform for artemisinin precursors. Vasilev, N. Comparison of plant-based expression platforms for the heterologous production of geraniol. Venter, M. Synthetic promoters: genetic control through cis engineering. Trends Plant Sci.

Customer Reviews

Von Schwartzenberg, K. The moss Physcomitrella patens releases a tetracyclic diterpene.


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